Кріоконсервування клітин-похідних нервового гребеня
DOI:
https://doi.org/10.15407/cryo35.01.003Ключові слова:
кріоконсервування, нервовий гребінь, 2D-культивування, 3D-культивування, клітини спінальних гангліїв, клітини пульпи зубів, меланоцити, шваннівські клітиниАнотація
Клітини-похідні нервового гребеня утворюються з нейроектодерми на ранніх стадіях ембріогенезу. Внаслідок епітеліо-мезенхімального переходу вони мігрують до периферичних структур та диференціюються у нейрони і глію периферичної нервової системи, меланоцити шкіри, клітини зубної пульпи, нейроендокринні клітини, хрящі та кістки черепа, а також у кілька інших фенотипів. Похідні нервового гребеня мають великий потенціал у регенеративній медицині. Кріоконсервування широко застосовується для довгострокового зберігання біологічного матеріалу, який в подальшому може бути використаний у клінічній практиці. У представленому огляді проаналізовано підходи до кріоконсервування клітин-похідних нервового гребеня, отриманих із різних джерел. Дослідження in vitro та in vivo демонструють успішність розроблених протоколів кріоконсервування різних клітин-похідних нервового гребеня, що дає можливість створення кріобанків та їх поширеного використання у клінічній практиці.
Probl Cryobiol Cryomed 2025; 35(1):3–13
Посилання
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