Problems of Cryobiology and Cryomedicine

Cover of the Issue

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Editorial Information

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Probl Cryobiol Cryomed 2024; 34(1):2

Thermal Field Monitoring When Exposing Soft Tissues to Low Temperatures: Thermography Prospects and Limitations

Sun, 19 May 2024 00:00:00 +0200

The review analyzes the existing tools for monitoring the dynamics of thermal fi elds when exposing the soft tissues to low temperatures. Features of contact and non-contact temperature measurements have been considered, their capabilities and limitations have been noted. There was substantiated the need to develop the procedures of intra-operative temperature control. Special attention has been paid to the non-contact non-invasive infrared thermography. This method has been shown to be applied for intra-operative monitoring of the movement of the ice lump edge on the surface of tissues, detection of a disordered thermal symmetry of the ice spot, thermal fi eld dynamics on the surface of tissues inside and outside the area of the operative zone. However, thermal imaging control of the dynamics of the primary necrosis zone and the ice ball edge in the volume of tissues is possible only under certain parameters of cryoimpact, for example, with a short-term cooling of tissues with a quasi-point nitrogen cryoapplicator. The possibility of using thermography at other stages of cryosurgery is also considered, i. e. as the method of additional diagnosis at the stage of surgery planning, as well as during the post-surgery period to control healing, scarring, etc.

Probl Cryobiol Cryomed 2024; 34(1):003–018

Amphiphilic Compounds as Antihemolytic Agents: Problems and Prospects

Sun, 19 May 2024 00:00:00 +0200

In this research the effect of surface-active substances belonging to different classes of amphiphilic compounds (cationic trifluoperazine (TFP), anionic sodium decyl sulfate (C10) and nonionic decyl-β,D-glucopyranoside (DGP)) on the level of damage of human erythrocytes during posthypertonic shock (PHS) and glycerol removal from cells frozen to –196°C was study. All amphiphilic compounds in low concentrations (at which the antihemolytic activity is approximately 45% under the conditions of PHS cells) show the same efficiency when removing glycerol from thawed cells. It was established that among amphiphilic compounds in effective concentrations the maximum antihemolytic (AHmax) activity was shown by DGP (74%) during the removal of glycerol from thawed cells and C10 (74%) under conditions of PHS of erythrocytes, while the AHmax activity indicators of TFP were comparable in both cases. A feature of the effectiveness of C10 under the conditions of deglycerolization of cryopreserved erythrocytes is almost the same AH when used in both concentrations. The method of flow cytometry showed that the amount of annexin-labeled cells depended on the concentration of C10 both in physiological solution and under PHS conditions.

Probl Cryobiol Cryomed 2024; 34(1):019–032

Cryopreserved Umbilical Cord Extract Restores the Skin Structure in Ovariectomized Rats

Sun, 19 May 2024 00:00:00 +0200

The research covers the specific effect of human umbilical cord cryoextract and the culture medium of its mesenchymal stromal cells in comparison with synthetic hyaluronic acid on the skin regeneration in female rats with simulated aging. The specified substances were administered intradermally to ovariectomized animals. The injection of human umbilical cord cryoextract into animals with a model of skin aging was established to restore the skin structure already 2 weeks after the injections. Thickening of the epidermis and dermis, phenomenon of cell proliferation, and an increased number of collagen fibers as well as blood vessels were observed. Four weeks after the last injection, the skin condition was normalized to the level of intact animals. Administration of the culture medium of mesenchymal stromal cells of the umbilical cord also led to an increased number of collagen fibers, cell proliferation, contouring of skin layers, but had a less pronounced effect compared to the injections of cryoextract on the indices of the dermis and epidermis thickness. Injections of synthetic hyaluronic acid led to "rejuvenation" of the upper layers of skin and had a less pronounced manifestation compared to the effect of human umbilical cord derivatives.

Probl Cryobiol Cryomed 2024; 34(1):033–044

Intra-Operative Infrared Thermography of Ocular Surface During Cryodestruction of Epibulbar Tumours

Oleg S. Zadorozhnyy, Oleksandr S. Buiko, Nataliya V. Pasyechnikova — Sun, 19 May 2024 00:00:00 +0200

The paper presents the possibilities of intraoperative use of infrared (IR) thermography to image the temperature distribution of the outer ocular surface during cryodestruction of conjunctival tumours with localization in the ciliary body projection to determine an individual freezing regimen. IR thermography was carried out using a portable device for infrared real-time thermography. Thermal fields were visualized, and the duration of cooling and warming of the structures of the outer ocular surface around the area of freezing, including the cornea, were recorded. It was found that intraoperative IR thermography during cryodestruction of epibulbar tumours in the projection of the ciliary body allows real-time noninvasive imaging of the temperature fi eld dynamics around the freezing zone, which can be used to select individual cooling exposure to reduce the risk of complications by avoiding excessive cooling of intraocular structures. It was also determined that IR thermography makes it possible to assess the individual dynamics of rewarming of the ocular tissues to the initial level if a repeated cycle of tumour cryodestruction is necessary.

Probl Cryobiol Cryomed 2024; 34(1):045–053

Dependence of Ascorbic Acid Content in Nettle Leaf Extracts on Cryoextraction Conditions

Ivan I. Shcheniavskyi, Yuliia S. Akhatova — Sun, 19 May 2024 00:00:00 +0200

Probl Cryobiol Cryomed 2024; 34(1):054–058

Information about the All-Ukrainian Scientific and Practical Conference ‘Stress-Induced Immune Disorders and Their Consequences in Wartime’

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Probl Cryobiol Cryomed 2024; 34(1):59-59

70th birthday of Oleksandr Petrenko

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Probl Cryobiol Cryomed 2024; 34(1):60-62

75th birthday of Tetiana Bondarenko

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Probl Cryobiol Cryomed 2024; 34(1):63-64

Instruction for Authors

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Probl Cryobiol Cryomed 2024; 34(1):67-68

Backmatter

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 19 May 2024 00:00:00 +0200

Problems of Cryobiology and Cryomedicine

Cover of the Issue

Editorial Information

Table of Contents

Thermal Field Monitoring When Exposing Soft Tissues to Low Temperatures: Thermography Prospects and Limitations

Amphiphilic Compounds as Antihemolytic Agents: Problems and Prospects

Cryopreserved Umbilical Cord Extract Restores the Skin Structure in Ovariectomized Rats

Intra-Operative Infrared Thermography of Ocular Surface During Cryodestruction of Epibulbar Tumours

Dependence of Ascorbic Acid Content in Nettle Leaf Extracts on Cryoextraction Conditions

Information about the All-Ukrainian Scientific and Practical Conference ‘Stress-Induced Immune Disorders and Their Consequences in Wartime’

70th birthday of Oleksandr Petrenko

75th birthday of Tetiana Bondarenko

Instruction for Authors

Backmatter